LaserStrobe time domain fluorometer
One of two elegant instruments in the lab used to obtain time-resolved fluorescence decay data. Pulsed excitation is from a nitrogen-pumped dye laser, with additional frequency doubling capability. The decay from the excited-state population of fluorophores is captured step-wise from time zero using a time-delayed gated photomultiplier. We have used these data in conjunction with fluorescence resonance energy transfer to obtain precise distance measurements for biomolecules in solution. Time-resolved fluorescence anisotropies give further information about probe and macromolecular rotation and mobility.
