1. Beck, J.R.; Harris, E.N. & Stains, C.I. Quantification of Cell Signaling Networks Using Kinase Activity Chemosensors. Methods Mol. Biol. 1636, 61-70 (2017). [Link]
  2. Zhou, X.; Lesiak, L.; Lai, R.; Beck, J.R.; Zhao, J.; Elowsky, C.G.; Li, H. & Stains, C.I. Chemoselective Alteration of Fluorophore Scaffolds as a Strategy for the Development of Ratiometric Chemodosimeters. Angew. Chem. Int. Ed. 56, 4197-4200 (2017). [Link]angew-toc
  3. Zhao, J. & Stains, C.I. Identification of a Fragmented Small GTPase Capable of Conditional Effector Binding. RSC Adv. 7, 12265-12268 (2017). [Link]


  1. Beck, J.R.; Truong, T. & Stains, C.I. Temporal Analysis of PP2A Phosphatase Activity During Insulin Stimulation Using a Direct Activity Probe. ACS Chem. Biol. 11, 3284-3288 (2016). [Link]toc-figure-1-1
  1. Zhou, X.; Lai, R.; Beck, J.R.; Li, H. & Stains, C.I. Nebraska Red: A Phosphinate-Based Near-Infrared Fluorophore Scaffold for Chemical Biology Applications. Chem. Commun. 52, 12290-12293 (2016). [Link]TOC_Neb Red
  2. Zhao, J.; Vu, Q. & Stains, C.I. Luminescent Platforms for Monitoring Changes in the Solubility of Amylin and Huntingtin in Living Cells. Mol. BioSyst. 12, 2984-2987 (2016). [Link]

Figure 1

  1. Xu, B.; Zhou, X. & Stains, C.I. An Improved Miniprotein Host for Fluorogenic Supramolecular Assembly on the Surface of Living Cells. RSC Adv. 6, 20381-20385 (2016). [Link]                                                                                       F
  2. Beck, J.R.; Lawrence, A.; Tung, A.S.; Harris, E.N. & Stains, C.I. Interrogating Endogenous Protein Phosphatase Activity with Rationally Designed Chemosensors. ACS Chem. Biol. 11, 284-290 (2016). [Link]T
  3. Zhao, J.; Nelson, T.J.; Vu, Q.; Truong, T. & Stains, C.I. Self-Assembling NanoLuc Luciferase Fragments as Probes for Protein Aggregation in Living Cells. ACS Chem. Biol. 11, 132-138 (2016). [Link]T
  4. Xu, B.; Zhou, X. & Stains, C.I. Supramolecular Assembly of an Evolved Miniprotein Host and Fluorogenic Guest Pair. J. Am. Chem. Soc137, 14252-14255 (2015). [Link]T
  5. Beck, J.R.; Zhou, X.; Casey, G.R. & Stains, C.I. Design and Evaluation of a Real-time Activity Probe for Focal Adhesion Kinase. Anal. Chim. Acta 897, 62-68 (2015). [Link]G
  6. Kelly, M.I.; Bechtel, T.J.; Reddy, D.R.; Hankore, E.D.; Beck, J.R. & Stains, C.I. A Real-Time, Fluorescence-Based Assay for Rho-Associated Protein Kinase Activity. Anal. Chim. Acta 891, 284-290 (2015). [Link]N
  7. Zhou, X.; Lai, R.; Li, H. & Stains, C.I. The 8-Silyloxyquinoline Scaffold as a Versatile Platform for the Sensitive Detection of Aqueous Fluoride. Anal. Chem. 87, 4081-4086 (2015). [Link]T
  8. Szalewski, D.A.; Beck, J.R. & Stains, C.I. Design, Synthesis, and Evaluation of a Selective Chemosensor for Leucine-rich Repeat Kinase 2. Bioorg. Med. Chem. Lett. 24, 5648-5651 (2014). [Link]N
  9. Beck, J.R.; Peterson, L.B.; Imperiali, B. & Stains, C.I. Quantification of Protein Kinase Enzymatic Activity in Unfractionated Cell Lysates Using CSox-Based Sensors. Curr. Protoc. Chem. Biol. 6, 135-156 (2014). [Link]

Prior to UNL

      1. Stains, C.I.; Tedford, N.C.; Walkup, T.C.; Luković, E.; Goguen, B.N.; Griffith, L.G.; Lauffenburger, D.A. & Imperiali, B. Interrogating Signaling Nodes Involved in Cellular Transformations Using Kinase Activity Probes. Chem. Biol. 19, 210-217 (2012).  [Link]
      1. Stains, C. I.; Luković, E. & Imperiali, B. A p38α-Selective Chemosensor for use in Unfractionated Cell Lysates. ACS Chem. Biol. 6, 101-105 (2011).  [Link]
      1. Furman, J. L.; Mok, P.; Shen, S.; Stains, C. I. & Ghosh, I. A Turn-on Split-Luciferase Sensor for the Direct Detection of Poly(ADP-Ribose) as a Marker for DNA Repair and Cell Death. Chem. Commun. 47, 397-399 (2011).  [Link]
      1. Stains, C. I.*; Furman, J. L.*; Porter, J. R.; Rajagopal, S.; Li, Y.; Wyatt, R. T. & Ghosh, I. A General Approach for Receptor and Antibody-Targeted Detection of Native Proteins Utilizing Split-Luciferase Reassembly. ACS Chem. Biol. 5, 943-952 (2010). *These authors contributed equally to this work.  [Link]
      1. Furman, J. L.; Badran, A. H.; Ajulo, O.; Porter, J. R.; Stains, C. I.; Segal, D. J. & Ghosh, I. Toward a General Approach for RNA-Templated Hierarchical Assembly of Split-Proteins. J. Am. Chem. Soc. 132, 11692-11701 (2010).  [Link]
      1. Furman, J. L.; Badran, A. H.; Shen, S.; Stains, C. I.; Hannallah, J.; Segal, D. J. & Ghosh, I. Systematic Evaluation of Split-Fluorescent Proteins for the Direct Detection of Native and Methylated DNA. Bioorg. Med. Chem. Lett. 19, 3748-3751 (2009).  [Link]
      1. Porter, J. R.; Stains, C. I.; Jester, B. & Ghosh, I. A General and Rapid Cell-Free Approach for the Interrogation of Protein-Protein, Protein-DNA, and Protein-RNA Interactions and their Antagonists Utilizing Split-Protein Reporters. J. Am. Chem. Soc. 130, 6488-6497 (2008).  [Link]
      1. Stains, C. I.; Mondal, K. & Ghosh, I. Molecules that Target Beta-Amyloid. ChemMedChem2, 1674-1692 (2007). [Link]
      1. Stains, C. I. & Ghosh, I. When Conjugated Polymers Meet Amyloid Fibrils. ACS Chem. Biol. 2, 525-528 (2007). [Link]
      1. Porter, J. R.; Stains, C. I.; Segal, D. J. & Ghosh, I. Split b-Lactamase Sensor for the Sequence-Specific Detection of DNA Methylation. Anal. Chem. 79, 6702-6708 (2007).  [Link]
      1. Smith, T. J.; Stains, C. I.; Meyer, S. C. & Ghosh, I. Inhibition of β-Amyloid Fibrillization by Directed Evolution of a β-Sheet Presenting Miniature Protein. J. Am. Chem. Soc. 128, 14456-14457 (2006).  [Link]
      1. Ghosh, I.; Stains, C. I.; Ooi, A. T. & Segal, D. J. Direct Detection of Double-Stranded DNA: Molecular Methods and Applications for DNA Diagnostics. Mol. BioSyst. 2, 551-560 (2006). [Link]
      1. Stains, C. I.; Furman, J. L.; Segal, D. J. & Ghosh, I. Site Specific Detection of DNA Methylation Utilizing mCpG-SEER. J. Am. Chem. Soc. 128, 9761-9765 (2006).  [Link]
      1. Ooi, A. T.; Stains, C. I.; Ghosh, I. & Segal, D. J. Sequence-Enabled Reassembly of β-Lactamase (SEER-LAC): A Sensitive Method for the Detection of Double-Stranded DNA. Biochemistry 46, 3620-3625 (2006). [Link]
      1. Ooi, A. T.; Stains, C. I.; Porter, J. R.; Ghosh, I. & Segal, D. J. Sequence-Enabled Reassembly (SEER) Peptides for the Detection of DNA Sequences. Proc. Am. Pep. Soc. 9, 214- 215 (2005).  [Link]
      1. Stains, C. I.; Porter, J. R.; Ooi, A. T.; Segal, D. J. & Ghosh, I. DNA Sequence-Enabled Reassembly of the Green Fluorescent Protein. J. Am. Chem. Soc. 127, 10782-10783 (2005). [Link]