Our laboratory is developing new fluorophores for applications in chemical biology. These scaffolds are being used to develop bioimaging reagents for proteins, small molecules, and post-translational modifications.
We are designing new host-guest pairs capable of functioning in biological systems. These new tools are being used to control biological functions through small-molecule-induced modulation of protein oligomerization in living cells.
Protein misfolding and aggregation plays a central role in numerous human diseases including Alzheimer’s, Parkinson’s, and Type II Diabetes. We have developed a sensitive luminescence-based assay that can be used to study this process in living cells as well as identify inhibitors.
Beck, J. R.; Lawrence, A.; Tung, A.; Harris, E. N. & Stains, C. I. Interrogating Endogenous Phosphatase Activity with Rationally Designed Chemosensors. ACS Chem. Biol.11, 284-290 (2016). DOI: 10.1021/acschembio.5b00506.
Zhao, J.; Nelson, T. J.; Vu, Q.; Truong, T. & Stains, C. I. Self-Assembling NanoLuc Luciferase Fragments as Probes for Protein Aggregation in Living Cells. ACS Chem. Biol.11, 132-138 (2016). DOI: 10.1021/acschembio.5b00758.
Xu, B.; Zhou, X. & Stains, C. I. Supramolecular Assembly of an Evolved Miniprotein Host and Fluorogenic Guest Pair. J. Am. Chem. Soc.,137, 14252-14255 (2015). DOI: 10.1021/jacs.5b09494